COMT - Antibodies - The Human Protein Atlas

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COMT

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA001169

Antibody CAB011233

Provider

Atlas Antibodies
Sigma-Aldrich

Origene

Product name

HPA001169

1671.00.02

Host species

Rabbit

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA001169, RRID:AB_1078402

VALIDATION SUMMARY

Antibody HPA001169

Antibody CAB011233

Immunocytochemistry

Immunohistochemistry

Supported

Western blot

Supported

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA001169

Antibody CAB011233

Formal validation: Tagged protein

Validated

Antibody staining overlaps with GFP tagged protein

Figure description

Immunofluorescent staining of transgenic HeLa cells show antibody staining in endoplasmic reticulum and GFP expression in endoplasmic reticulum.

Antibody dilution

1:8

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.

Antibody dilution

1:15

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA001169

Antibody CAB011233

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.

Immunohistochemical staining of human placenta shows strong cytoplasmic and nuclear positivity in cytotrophoblasts.

Expression

RNA: detected in 37 tissues
Protein: detected in 68 cell types

RNA: detected in 37 tissues
Protein: detected in 66 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

1:5000

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA001169

Antibody CAB011233

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17.1
Lane 2: RT4
Lane 3: EFO-21
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

30, 24.4

30, 26, 24.8, 24.4, 16.8

Antibody dilution

1:500

PROTEIN ARRAY

Antibody HPA001169

Antibody CAB011233

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA001169

Antibody CAB011233

Antigen

Recombinant protein fragment

Genetic immunization

Length (aa)

181

Antigen sequence

YCEQKEWAMNVGDKKGKIVDAVIQEHQPSVLLELGAYCGYSAVRMARLLS
PGARLITIEINPDCAAITQRMVDFAGVKDKVTLVVGASQDIIPQLKKKYD
VDTLDMVFLDHWKDRYLPDTLLLEECGLLRKGTVLLADNVICPGAPDFLA
HVRGSSCFECTHYQSFLEYREVVDGLEKAIY

Matching transcripts

COMT-001 - ENSP00000385150 [100%]
COMT-002 - ENSP00000385917 [100%]
COMT-003 - ENSP00000384654 [100%]
COMT-011 - ENSP00000354511 [100%]
COMT-012 - ENSP00000416778 [100%]

Other gene match

ANTIGEN VIEW