Fields »
ABOUT
HELP
BLOG
LRRFIP2

ANTIBODY INFORMATION
 
 

Antibody HPA035956

  

Antibody HPA042023

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA035956   HPA042023  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   12  

References

    

Proper citation

 Atlas Antibodies Cat#HPA035956, RRID:AB_10671238   Atlas Antibodies Cat#HPA042023, RRID:AB_2677802  

VALIDATION SUMMARY
 
 

Antibody HPA035956

  

Antibody HPA042023

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Uncertain    
Western blot

Uncertain   Supported  
Protein array

Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA035956

  

Antibody HPA042023

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & actin filaments.  

Antibody dilution

   1:20  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA035956

  

Antibody HPA042023

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 65 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:300    

Literature conformity

 Partly consistent with gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA035956

  

Antibody HPA042023

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 82.2   82.2, 57.4, 48.3, 45.4, 20.9  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA035956

  

Antibody HPA042023

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:30000   1:12000  

ANTIGEN INFORMATION
 
 

Antibody HPA035956

  

Antibody HPA042023

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 88   92  

Antigen sequence

 
PGFTNDDTASIVSSDRASRGRRESVVSAADYFSRSNRRGSVVSEVDDISI
PDLSSLDEKSDKQYAENYTRPSSRNSASATTPLSGNSS
  
VKTGEKHGLVIIPDGTPNGDVSHEPVAGAITVVSQEAAQVLESAGEGPLD
VRLRKLAGEKEELLSQIRKLKLQLEEERQKCSRNDGTVGDLA
 

Matching transcripts

 LRRFIP2-001 - ENSP00000338727 [100%]
  LRRFIP2-001 - ENSP00000338727 [100%]
LRRFIP2-002 - ENSP00000346349 [96%]
LRRFIP2-003 - ENSP00000416364 [96%]
LRRFIP2-004 - ENSP00000405480 [96%]
LRRFIP2-006 - ENSP00000379705 [95%]
LRRFIP2-023 - ENSP00000413026 [90%]
 

Other gene match

    
ANTIGEN VIEW
LRRFIP2-001
LRRFIP2-002
LRRFIP2-003
LRRFIP2-004
LRRFIP2-006
LRRFIP2-023
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.