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Immunohistochemical staining of human hair shows strong cytoplasmic positivity in cells in cortex/medulla.
Immunohistochemical staining of human hair shows strong cytoplasmic positivity in cells in hair cortex.
Expression
RNA: detected in 4 tissues Protein: detected in 0 cell types
RNA: detected in 4 tissues Protein: detected in 0 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:900
1:2000
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA049550
Antibody HPA055194
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
47.2
49.4, 47.2, 46.2, 45.9
Antibody dilution
1:490
1:270
PROTEIN ARRAY
Antibody HPA049550
Antibody HPA055194
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
