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Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane.
Antibody dilution
1:132
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA040086
Standard validation
Approved
Figure description
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in non-germinal center cells.
Expression
RNA: detected in 37 tissues Protein: detected in 45 cell types
Retrieval
HIER pH6
Antibody dilution
1:300
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA040086
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402460)
Target mass (kDa)
59.6, 55.8, 45.6
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA040086
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
