Fields »
ABOUT
HELP
BLOG
MLN

ANTIBODY INFORMATION
 
 

Antibody HPA069392

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA069392  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 14  

References

  

Proper citation

 Atlas Antibodies Cat#HPA069392, RRID:AB_2686136  

VALIDATION SUMMARY
 
 

Antibody HPA069392

  
Immunohistochemistry


Validated
  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA069392

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in enteroendocrine cells.  

Expression

 RNA: detected in 2 tissues
Protein: detected in 2 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:1500  

Literature conformity

 Partly consistent with gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA069392

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

 

Figure description

  

Target mass (kDa)

 12.9, 12.8, 12.1  

Antibody dilution

 1:450  

PROTEIN ARRAY
 
 

Antibody HPA069392

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:5400  

ANTIGEN INFORMATION
 
 

Antibody HPA069392

  

Antigen

 Recombinant protein fragment  

Length (aa)

 50  

Antigen sequence

 
AFVPIFTYGELQRMQEKERNKGQKKSLSVWQRSGEEGPVDPAEPIREEEN
 

Matching transcripts

 MLN-001 - ENSP00000388825 [100%]
MLN-002 - ENSP00000425467 [100%]
MLN-003 - ENSP00000266003 [100%]
 

Other gene match

  
ANTIGEN VIEW
MLN-001
MLN-002
MLN-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.