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Immunofluorescent staining of human cell line SH-SY5Y shows localization to vesicles.
Antibody dilution
1:85
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA051805
Antibody HPA055106
Antibody CAB015467
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human duodenum shows strong luminal membranous positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.
Immunohistochemical staining of human colon shows strong luminal membranous positivity in glandular cells.
Expression
RNA: detected in 23 tissues Protein: detected in 20 cell types
RNA: detected in 23 tissues Protein: detected in 5 cell types
RNA: detected in 23 tissues Protein: detected in 10 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:1400
1:1700
1:1000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA051805
Antibody HPA055106
Antibody CAB015467
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
73.5, 60.1
73.5, 60.1
73.5, 60.1
Antibody dilution
1:240
1:430
1:500
PROTEIN ARRAY
Antibody HPA051805
Antibody HPA055106
Antibody CAB015467
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
