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ANTIBODY INFORMATION
 
 

Antibody HPA032113

  

Antibody HPA032114

  

Antibody CAB034296

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA032113   HPA032114   sc-86450  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 13   7   8  

References

   1    

Proper citation

 Atlas Antibodies Cat#HPA032113, RRID:AB_2674156   Atlas Antibodies Cat#HPA032114, RRID:AB_10696202   Santa Cruz Biotechnology Cat#sc-86450, RRID:AB_2102563  

VALIDATION SUMMARY
 
 

Antibody HPA032113

  

Antibody HPA032114

  

Antibody CAB034296

  
Immunocytochemistry

Supported      
Immunohistochemistry

  Uncertain   Uncertain  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA032113

  

Antibody HPA032114

  

Antibody CAB034296

  

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus & cytosol.      

Antibody dilution

 1:200      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA032113

  

Antibody HPA032114

  

Antibody CAB034296

  

Standard validation

   Uncertain   Uncertain  

     

Figure description

   Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.   Immunohistochemical staining of human bronchus shows moderate cytoplasmic positivity in respiratory epithelial cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 75 cell types		   RNA: detected in 37 tissues
Protein: detected in 32 cell types		  

Retrieval

   HIER pH6   HIER pH6  

Antibody dilution

   1:800   1:85  

Literature conformity

   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA032113

  

Antibody HPA032114

  

Antibody CAB034296

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 58.5   58.5, 49.4, 45.8   58.5, 49.4, 45.8, 5.9  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA032113

  

Antibody HPA032114

  

Antibody CAB034296

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:30000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA032113

  

Antibody HPA032114

  

Antibody CAB034296

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 87   77    

Antigen sequence

 
PTLGHLRSHLRQSLLCTWGYSSNTRFTITLNYKDPLTGDEETLASYGIVS
GDLICLILQDDIPAPNIPSSTDSEHSSLQNNEQPSLA
  
EGTGFYPSEPMLCSESVEGQVPHSLETLYQSADCSDANDALIVLIHLLML
ESGYIPQGTEAKALSMPEKWKLSGVYK
  

											 
																				

																		

										
											
Matching transcripts

												
																					FBXO7-001 - ENSP00000266087 [100%] 

											 
																						FBXO7-001 - ENSP00000266087 [100%] 
FBXO7-002 - ENSP00000380571 [100%] 
FBXO7-006 - ENSP00000388547 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
FBXO7-001
FBXO7-002
FBXO7-006
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA032113

												
										 
																				
											
											
											
Antibody HPA032114

												
										 
																				
											
											
											
Antibody CAB034296

												
										 
																		

																	

										
											
1.

												
																					
											 
																							Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

	Stadler C et al

	Nat Methods 2013;10(4):315-23

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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