PACSIN2 - Antibodies - The Human Protein Atlas

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PACSIN2

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA049854

Antibody HPA056520

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA049854

HPA056520

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA049854, RRID:AB_2680915

Atlas Antibodies Cat#HPA056520, RRID:AB_2683163

VALIDATION SUMMARY

Antibody HPA049854

Antibody HPA056520

Immunocytochemistry

Supported

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA049854

Antibody HPA056520

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line CACO-2 shows localization to nuclear speckles, plasma membrane, cytosol & vesicles.

Antibody dilution

1:6

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA049854

Antibody HPA056520

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA056520 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA049854 across 46 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in renal tubules.

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.

Expression

RNA: detected in 37 tissues
Protein: detected in 54 cell types

RNA: detected in 37 tissues
Protein: detected in 53 cell types

Retrieval

HIER pH6

Antibody dilution

1:450

1:1200

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA049854

Antibody HPA056520

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

55.7, 51.8

55.7, 51.8, 51.4

Antibody dilution

1:80

1:330

PROTEIN ARRAY

Antibody HPA049854

Antibody HPA056520

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:3950

ANTIGEN INFORMATION

Antibody HPA049854

Antibody HPA056520

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

NVPSNPAQSAQSQSSYNPFEDEDDTGSTVSEKDDTKAKNVSSYEKTQSYP
TDWS

FEEKRLRFFREVLLEVQKHLDLSNVAGYKAIYHDLEQSIRAADAVEDLR

Matching transcripts

PACSIN2-001 - ENSP00000263246 [100%]
PACSIN2-004 - ENSP00000385040 [100%]
PACSIN2-005 - ENSP00000385372 [100%]
PACSIN2-016 - ENSP00000489297 [100%]

PACSIN2-001 - ENSP00000263246 [100%]
PACSIN2-003 - ENSP00000385952 [100%]
PACSIN2-004 - ENSP00000385040 [100%]
PACSIN2-005 - ENSP00000385372 [100%]
PACSIN2-016 - ENSP00000489297 [100%]
PACSIN2-201 - ENSP00000338379 [100%]

Other gene match

ANTIGEN VIEW

PACSIN2-001	PACSIN2-003	PACSIN2-004	PACSIN2-005	PACSIN2-016	PACSIN2-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.