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AP1B1

ANTIBODY INFORMATION
 
 

Antibody HPA065226

  

Antibody HPA068520

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA065226   HPA068520  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 14   16  

References

    

Proper citation

 Atlas Antibodies Cat#HPA065226, RRID:AB_2685438   Atlas Antibodies Cat#HPA068520, RRID:AB_2685998  

VALIDATION SUMMARY
 
 

Antibody HPA065226

  

Antibody HPA068520

  
Immunocytochemistry

Supported    
Immunohistochemistry

  Approved  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA065226

  

Antibody HPA068520

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol & the Golgi apparatus.    

Antibody dilution

 1:200    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA065226

  

Antibody HPA068520

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 70 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:175  

Literature conformity

   Consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA065226

  

Antibody HPA068520

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 104.6, 103.5, 101.3   104.6, 103.5, 101.3  

Antibody dilution

 1:1140   1:140  

PROTEIN ARRAY
 
 

Antibody HPA065226

  

Antibody HPA068520

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:30000   1:1700  

ANTIGEN INFORMATION
 
 

Antibody HPA065226

  

Antibody HPA068520

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 39   39  

Antigen sequence

 
PPSAFVEGGRGVVHKSLPPRTASSESAESPETAPTGAPP
  
GGLDSLMGDEPEGIGGTNFVAPPTAAVPANLGAPIGSGL
 

Matching transcripts

 AP1B1-001 - ENSP00000384194 [100%]
AP1B1-002 - ENSP00000319361 [100%]
AP1B1-004 - ENSP00000386071 [100%]
AP1B1-201 - ENSP00000350199 [100%]
AP1B1-202 - ENSP00000387612 [100%]
AP1B1-203 - ENSP00000400065 [100%]
  AP1B1-001 - ENSP00000384194 [100%]
AP1B1-201 - ENSP00000350199 [100%]
AP1B1-002 - ENSP00000319361 [82%]
AP1B1-004 - ENSP00000386071 [82%]
AP1B1-202 - ENSP00000387612 [82%]
AP1B1-203 - ENSP00000400065 [82%]
 

Other gene match

    
ANTIGEN VIEW
AP1B1-001
AP1B1-002
AP1B1-004
AP1B1-201
AP1B1-202
AP1B1-203
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.