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Immunofluorescent staining of human cell line MCF7 shows localization to vesicles.
Antibody dilution
1:75
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA011972
Antibody CAB011604
Antibody CAB018341
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic positivity in granular pattern in neuronal cells.
Immunohistochemical staining of human skeletal muscle shows distinct cytoplasmic positivity in myocytes.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet cells.
Expression
RNA: detected in 36 tissues Protein: detected in 73 cell types
RNA: detected in 36 tissues Protein: detected in 72 cell types
RNA: detected in 36 tissues Protein: detected in 69 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:30
1:500
1:65
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA011972
Antibody CAB011604
Antibody CAB018341
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400920)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
27.3, 25.5, 16.3, 15.5
27.3, 25.5, 16.3, 15.5
27.3, 25.5, 16.3, 15.5
Antibody dilution
1:250
1:500
1:500
PROTEIN ARRAY
Antibody HPA011972
Antibody CAB011604
Antibody CAB018341
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
