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Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in a subset of lymphoid cells outside reaction center.
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.
Expression
RNA: detected in 29 tissues Protein: detected in 7 cell types
RNA: detected in 29 tissues Protein: detected in 8 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:200
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003418
Antibody CAB000376
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401332)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
27.7, 26.4, 22.7, 17
27.7, 26.4, 22.7, 17, 10.2
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA003418
Antibody CAB000376
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
