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ANTIBODY INFORMATION
 
 

Antibody HPA017369

  

Antibody CAB000023

  

Antibody CAB040544

  

Antibody CAB055506

  

Antibody CAB058688

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Roche
   NCI-CPTC
   DakoCytomation
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA017369   1199 021   CPTC-CHGA-2   M0869   AMAb90525  

Host species

 Rabbit   Mouse   Mouse   Mouse   Mouse  

Clonality

 pAb   mAb   mAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known   Protein A/G   Supernatant   Protein A/G  

Other gene match

          

Released in version

 4   1   7   11   12  

References

 3          

Proper citation

 Atlas Antibodies Cat#HPA017369, RRID:AB_1846698       Dako Cat#M0869, RRID:AB_2081135   Atlas Antibodies Cat#AMAb90525, RRID:AB_2665574  

VALIDATION SUMMARY
 
 

Antibody HPA017369

  

Antibody CAB000023

  

Antibody CAB040544

  

Antibody CAB055506

  

Antibody CAB058688

  
Immunocytochemistry

Approved          
Immunohistochemistry


Validated
  

Validated
  

Validated
  

Validated
  

Validated
  
Western blot

Supported   Supported   Uncertain   Uncertain   Uncertain  
Protein array

Supported          

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA017369

  

Antibody CAB000023

  

Antibody CAB040544

  

Antibody CAB055506

  

Antibody CAB058688

  

Standard validation

 Approved          

         

Figure description

 Immunofluorescent staining of human cell line SH-SY5Y shows localization to vesicles.          

Antibody dilution

 1:50          

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.          

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA017369

  

Antibody CAB000023

  

Antibody CAB040544

  

Antibody CAB055506

  

Antibody CAB058688

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
 
 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 
 
 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported   Supported   Supported   Supported   Supported  

         

Figure description

 Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in subset of glandular cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet cells.   Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in enterochromaffin cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.  

Expression

 RNA: detected in 15 tissues
Protein: detected in 19 cell types		   RNA: detected in 15 tissues
Protein: detected in 17 cell types		   RNA: detected in 15 tissues
Protein: detected in 17 cell types		   RNA: detected in 15 tissues
Protein: detected in 12 cell types		   RNA: detected in 15 tissues
Protein: detected in 21 cell types		  

Retrieval

 HIER pH6   HIER pH9   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:600   1:5000   1:275   1:2000   1:2000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA017369

  

Antibody CAB000023

  

Antibody CAB040544

  

Antibody CAB055506

  

Antibody CAB058688

  

Standard validation

 Supported

Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

         

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400511)		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 50.7   50.7, 34.3   50.7, 34.3   50.7, 34.3   50.7, 34.3  

Antibody dilution

 1:250   1:500   1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA017369

  

Antibody CAB000023

  

Antibody CAB040544

  

Antibody CAB055506

  

Antibody CAB058688

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		          

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.          

Antibody dilution

 1:3000          

ANTIGEN INFORMATION
 
 

Antibody HPA017369

  

Antibody CAB000023

  

Antibody CAB040544

  

Antibody CAB055506

  

Antibody CAB058688

  

Antigen

 Recombinant protein fragment   Not known   Recombinant protein   Native protein   Recombinant protein  

Length (aa)

 141          

Antigen sequence

 
NSPMNKGDTEVMKCIVEVISDTLSKPSPMPVSQECFETLRGDERILSILR
HQNLLKELQDLALQGAKERAHQQKKHSGFEDELSEVLENQSSQAELKEAV
EEPSSKDVMEKREDSKEAEKSGEATDGARPQALPEPMQESK
  

											 
																						

											 
																						

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					CHGA-001 - ENSP00000216492 [100%] 

											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CHGA-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA017369

												
										 
																				
											
											
											
Antibody CAB000023

												
										 
																				
											
											
											
Antibody CAB040544

												
										 
																				
											
											
											
Antibody CAB055506

												
										 
																				
											
											
											
Antibody CAB058688

												
										 
																		

																	

										
											
1.

												
																						Scalable in situ hybridization on tissue arrays for validation of novel cancer and tissue-specific biomarkers

	Kiflemariam S et al

	PLoS One 2012;7(3):e32927

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Identification of a gene regulatory network associated with prion replication

	Marbiah MM et al

	EMBO J 2014;33(14):1527-47

			Application: ICC-IF				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
3.

												
																						A recellularized human colon model identifies cancer driver genes

	Chen HJ et al

	Nat Biotechnol 2016;34(8):845-51

			Application: ICC-IF				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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