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CD40

ANTIBODY INFORMATION
 
 

Antibody HPA031567

  

Antibody HPA031568

  

Antibody CAB002495

  

Antibody CAB072868

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Novocastra
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA031567   HPA031568   NCL-CD40   AMAb90905  

Host species

 Rabbit   Rabbit   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known   Protein A/G  

Other gene match

        

Released in version

 12   12   1   14  

References

        

Proper citation

 Atlas Antibodies Cat#HPA031567, RRID:AB_2673936   Atlas Antibodies Cat#HPA031568, RRID:AB_2673937     Atlas Antibodies Cat#AMAb90905, RRID:AB_2665719  

VALIDATION SUMMARY
 
 

Antibody HPA031567

  

Antibody HPA031568

  

Antibody CAB002495

  

Antibody CAB072868

  
Immunohistochemistry

Supported   Supported   Supported  

Validated
  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA031567

  

Antibody HPA031568

  

Antibody CAB002495

  

Antibody CAB072868

  

Formal validation: Orthogonal

      
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

     
 

Figure description

       Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human appendix shows cytoplasmic positivity in lymphoid cells.   Immunohistochemical staining of human appendix shows cytoplasmic positivity in lymphoid cells.   Immunohistochemical staining of human spleen shows moderate cytoplasmic positivity in cells in white pulp.   Immunohistochemical staining of human spleen shows cytoplasmic positivity in white pulp.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 14 cell types		   RNA: detected in 37 tissues
Protein: detected in 23 cell types		   RNA: detected in 37 tissues
Protein: detected in 6 cell types		   RNA: detected in 37 tissues
Protein: detected in 15 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH9   HIER pH6  

Antibody dilution

 1:600   1:150   1:20   1:1500  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA031567

  

Antibody HPA031568

  

Antibody CAB002495

  

Antibody CAB072868

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

       

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 30.6, 22.3, 17   30.6   30.6, 22.3, 17   30.6, 22.3, 17  

Antibody dilution

 1:250   1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA031567

  

Antibody HPA031568

  

Antibody CAB002495

  

Antibody CAB072868

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000   1:500      

ANTIGEN INFORMATION
 
 

Antibody HPA031567

  

Antibody HPA031568

  

Antibody CAB002495

  

Antibody CAB072868

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known   Recombinant protein  

Length (aa)

 78   84      

Antigen sequence

 
KQYLINSQCCSLCQPGQKLVSDCTEFTETECLPCGESEFLDTWNRETHCH
QHKYCDPNLGLRVQQKGTSETDTICTCE
  
EGWHCTSEACESCVLHRSCSPGFGVKQIATGVSDTICEPCPVGFFSNVSS
AFEKCHPWTSCETKDLVVQQAGTNKTDVVCGPQD
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					CD40-001 - ENSP00000361359 [100%] 
CD40-002 - ENSP00000361350 [100%] 
CD40-201 - ENSP00000484074 [100%] 

											 
																						CD40-001 - ENSP00000361359 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CD40-001
CD40-002
CD40-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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