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ANTIBODY INFORMATION
 
 

Antibody HPA061284

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA061284  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 13  

References

  

Proper citation

 Atlas Antibodies Cat#HPA061284, RRID:AB_2684465  

VALIDATION SUMMARY
 
 

Antibody HPA061284

  
Immunohistochemistry


Validated
  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA061284

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human bone marrow shows distinct cytoplasmic positivity in subsets of hematopoietic cells.  

Expression

 RNA: detected in 20 tissues
Protein: detected in 5 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:800  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA061284

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

 

Figure description

  

Target mass (kDa)

 53.9, 31.9  

Antibody dilution

 1:300  

PROTEIN ARRAY
 
 

Antibody HPA061284

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3550  

ANTIGEN INFORMATION
 
 

Antibody HPA061284

  

Antigen

 Recombinant protein fragment  

Length (aa)

 81  

Antigen sequence

 
TTGSMEVSAESNRLVGELKLDRLLLELKHSNIGPFPVELLQDIMNYIVPI
LVLPRVNEKLQKGFPLPTPARVQLYNVVLQP
 

Matching transcripts

 BPI-001 - ENSP00000409833 [100%]
BPI-002 - ENSP00000262865 [100%]
 

Other gene match

  
ANTIGEN VIEW
BPI-001
BPI-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.