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ANTIBODY INFORMATION
 
 

Antibody HPA041225

  

Antibody HPA044675

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA041225   HPA044675  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   11  

References

   1  

Proper citation

 Atlas Antibodies Cat#HPA041225, RRID:AB_2677371   Atlas Antibodies Cat#HPA044675, RRID:AB_2679041  

VALIDATION SUMMARY
 
 

Antibody HPA041225

  

Antibody HPA044675

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Supported  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA041225

  

Antibody HPA044675

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line CACO-2 shows localization to cytosol.  

Antibody dilution

   1:68  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041225

  

Antibody HPA044675

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.   Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 74 cell types		   RNA: detected in 37 tissues
Protein: detected in 55 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:15   1:350  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041225

  

Antibody HPA044675

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 47.7, 44.7   47.7, 44.7  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA041225

  

Antibody HPA044675

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA041225

  

Antibody HPA044675

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 87   78  

Antigen sequence

 
MEGYEVTTMDEACQEGNIFVTTTGCIDIILGRHFEQMKDDAIVCNIGHFD
VEIDVKWLNENAVEKVNIKPQVDRYRLKNGRRIILLA
  
HPSFVMSNSFTNQVMAQIELWTHPDKYPVGVHFLPKKLDEAVAEAHLGKL
NVKLTKLTEKQAQYLGMSCDGPFKPDHY
 

Matching transcripts

 AHCY-002 - ENSP00000217426 [100%]
AHCY-006 - ENSP00000442820 [100%]
  AHCY-002 - ENSP00000217426 [100%]
AHCY-006 - ENSP00000442820 [100%]
 

Other gene match

    
ANTIGEN VIEW
AHCY-002
AHCY-006

RELEVANT PUBLICATIONS
 
 

Antibody HPA041225

  

Antibody HPA044675

  

1.

   RNA deep sequencing as a tool for selection of cell lines for systematic subcellular localization of all human proteins
Danielsson F et al
J Proteome Res 2013;12(1):299-307
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.