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MYOM1

ANTIBODY INFORMATION
 
 

Antibody HPA014305

  

Antibody HPA049193

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA014305   HPA049193  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   13  

References

    

Proper citation

 Atlas Antibodies Cat#HPA014305, RRID:AB_1854257   Atlas Antibodies Cat#HPA049193, RRID:AB_2680670  

VALIDATION SUMMARY
 
 

Antibody HPA014305

  

Antibody HPA049193

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA014305

  

Antibody HPA049193

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.   Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.  

Expression

 RNA: detected in 32 tissues
Protein: detected in 9 cell types		   RNA: detected in 32 tissues
Protein: detected in 10 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:3500   1:60  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA014305

  

Antibody HPA049193

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 187.6, 177.7, 162.3   187.6, 177.7, 162.3  

Antibody dilution

 1:250   1:240  

PROTEIN ARRAY
 
 

Antibody HPA014305

  

Antibody HPA049193

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:1950  

ANTIGEN INFORMATION
 
 

Antibody HPA014305

  

Antibody HPA049193

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 145   100  

Antigen sequence

 
GITDTEEERIKEAAAYIAQRNLLASEEGITTPKQSTASKQTTASKQSTAS
KQSTASKQSTASRQSTASRQSVVSKQATSALQQEETSEKKSRKVVIREKA
ERLSLRKTLEETETYHAKLNEDHLLHAPEFIIKPRSHTVWEKENV
  
FDETRFHAGASTMPLSFGVTPYGYASRFEIHFDDKFDVSFGREGETMSLG
CRVVITPEIKHFQPEIQWYRNGVPLSPSKWVQTLWSGERATLTFSHLNKE
 

Matching transcripts

 MYOM1-001 - ENSP00000348821 [99%]
MYOM1-003 - ENSP00000261606 [99%]
MYOM1-201 - ENSP00000383413 [99%]
  MYOM1-001 - ENSP00000348821 [100%]
MYOM1-003 - ENSP00000261606 [100%]
MYOM1-201 - ENSP00000383413 [100%]
 

Other gene match

    
ANTIGEN VIEW
MYOM1-001
MYOM1-003
MYOM1-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.