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CSTF2

ANTIBODY INFORMATION
 
 

Antibody HPA000427

  

Antibody CAB004680

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Abnova
  

Product name

 HPA000427   H00001478-M01  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 1   2  

References

    

Proper citation

 Atlas Antibodies Cat#HPA000427, RRID:AB_1078580   Abnova Corporation Cat#H00001478-M01, RRID:AB_425385  

VALIDATION SUMMARY
 
 

Antibody HPA000427

  

Antibody CAB004680

  
Immunocytochemistry


Validated
    
Immunohistochemistry

Supported   Supported  
Western blot


Validated
   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000427

  

Antibody CAB004680

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		    

   

Figure description

 Nuclear region of segmented cells in 10x-images    

Antibody dilution

 1:90    
 

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.    

Antibody dilution

 1:91    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000427

  

Antibody CAB004680

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.   Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 67 cell types		   RNA: detected in 36 tissues
Protein: detected in 66 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:750   1:600  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA000427

  

Antibody CAB004680

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		    

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		    

Target mass (kDa)

 62.9, 61    

Loading control

 Total protein image    

Antibody dilution

 1:200    
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17.1
Lane 2: RT4
Lane 3: EFO-21
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 62.9, 61   62.9, 61, 24.7  

Antibody dilution

 1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA000427

  

Antibody CAB004680

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA000427

  

Antibody CAB004680

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 119    

Antigen sequence

 
GRGGRDPRGIDARGMEARAMEARGLDARGLEARAMEARAMEARAMEARAM
EARAMEVRGMEARGMDTRGPVPGPRGPIPSGMQGPSPINMGAVVPQGSRQ
VPVMQGTGMQGASIQGGSQ
  

											 
																				

																		

										
											
Matching transcripts

												
																					CSTF2-001 - ENSP00000362063 [100%] 
CSTF2-201 - ENSP00000387996 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CSTF2-001
CSTF2-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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