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Immunohistochemical staining of human parathyroid gland shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in renal tubules.
Expression
RNA: detected in 36 tissues Protein: detected in 41 cell types
RNA: detected in 36 tissues Protein: detected in 27 cell types
Retrieval
HIER pH9
HIER pH6
Antibody dilution
1:20
1:200
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA000226
Antibody HPA000250
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407971)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407971)
Target mass (kDa)
52.3, 52.2, 52, 51.2, 43.5
52.3, 52.2, 52, 51.2
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA000226
Antibody HPA000250
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.