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GPR50

ANTIBODY INFORMATION
 
 

Antibody HPA049862

  

Antibody HPA054678

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA049862   HPA054678  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   16  

References

    

Proper citation

 Atlas Antibodies Cat#HPA049862, RRID:AB_2680920   Atlas Antibodies Cat#HPA054678, RRID:AB_2682570  

VALIDATION SUMMARY
 
 

Antibody HPA049862

  

Antibody HPA054678

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA049862

  

Antibody HPA054678

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane.  

Antibody dilution

   1:23  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA049862

  

Antibody HPA054678

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.   Immunohistochemical staining of human pituitary gland shows strong cytoplasmic positivity in cells in anterior lobe.  

Expression

 RNA: detected in 0 tissues
Protein: detected in 54 cell types		   RNA: detected in 0 tissues
Protein: detected in 11 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:400  

Literature conformity

 Not consistent with gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA049862

  

Antibody HPA054678

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 67.4, 67.3   67.4, 67.3  

Antibody dilution

 1:250   1:120  

PROTEIN ARRAY
 
 

Antibody HPA049862

  

Antibody HPA054678

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3500   1:950  

ANTIGEN INFORMATION
 
 

Antibody HPA049862

  

Antibody HPA054678

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 80   47  

Antigen sequence

 
RHPIIFFSGLISDIREMQEARTLARARAHARDQAREQDRAHACPAVEETP
MNVRNVPLPGDAAAGHPDRASGHPKPHSRS
  
PTKPAASQLESDTIADLPDPTVVTTSTNDYHDVVVIDVEDDPDEMAV
 

Matching transcripts

 GPR50-001 - ENSP00000218316 [100%]
GPR50-201 - ENSP00000484496 [100%]
  GPR50-001 - ENSP00000218316 [100%]
GPR50-201 - ENSP00000484496 [100%]
 

Other gene match

    
ANTIGEN VIEW
GPR50-001
GPR50-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.