We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Antibody staining overlaps with antibody HPA031573.
Validated
Antibody staining overlaps with antibody HPA031572.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli & cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli & cytosol.
Antibody dilution
1:57
1:200
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA031572
Antibody HPA031573
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA031573 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA031572 across 46 cell lines.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human small intestine shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 79 cell types
RNA: detected in 37 tissues Protein: detected in 78 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:30
1:350
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA031572
Antibody HPA031573
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415179)
Target mass (kDa)
65, 63.1
65, 63.1, 55.8
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA031572
Antibody HPA031573
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.