RBM3 - Antibodies - The Human Protein Atlas

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RBM3

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA003624

Antibody CAB062559

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA003624

AMAb90655

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA003624, RRID:AB_10602123

Atlas Antibodies Cat#AMAb90655, RRID:AB_2665621

VALIDATION SUMMARY

Antibody HPA003624

Antibody CAB062559

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA003624

Antibody CAB062559

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Antibody dilution

1:35

1:200

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA003624

Antibody CAB062559

Standard validation

Approved

Figure description

Immunohistochemical staining of human skin shows moderate nuclear positivity in epidermal cells.

Immunohistochemical staining of human esophagus shows moderate nuclear positivity in squamous epithelial cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 14 cell types

RNA: detected in 37 tissues
Protein: detected in 36 cell types

Retrieval

HIER pH6

Antibody dilution

1:150

1:1000

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA003624

Antibody CAB062559

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

17.2

17.2, 16.7

Antibody dilution

1:250

1:1000

PROTEIN ARRAY

Antibody HPA003624

Antibody CAB062559

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA003624

Antibody CAB062559

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

134

Antigen sequence

DEQALEDHFSSFGPISEVVVVKDRETQRSRGFGFITFTNPEHASVAMRAM
NGESLDGRQIRVDHAGKSARGTRGGGFGAHGRGRSYSRGGGDQGYGSGRY
YDSRPGGYGYGYGRSRDYNGRNQGGYDRYSGGNY

Matching transcripts

RBM3-003 - ENSP00000365950 [100%]
RBM3-005 - ENSP00000365946 [100%]

Other gene match

ANTIGEN VIEW

RBM3-003	RBM3-005

RELEVANT PUBLICATIONS

Antibody HPA003624

Antibody CAB062559

Nuclear expression of the RNA-binding protein RBM3 is associated with an improved clinical outcome in breast cancer
Jögi A et al
Mod Pathol 2009;22(12):1564-74

High nuclear RBM3 expression is associated with an improved prognosis in colorectal cancer
Hjelm B et al
Proteomics Clin Appl 2011;5(11-12):624-35

Generation of monospecific antibodies based on affinity capture of polyclonal antibodies
Hjelm B et al
Protein Sci 2011;20(11):1824-35

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.