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Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & cytosol.
Antibody dilution
1:77
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA045105
Antibody HPA051935
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human breast shows strong cytoplasmic and membranous positivity in glandular cells along with distinct extracellular positivity..
Expression
RNA: detected in 37 tissues Protein: detected in 47 cell types
Retrieval
HIER pH6
Antibody dilution
1:900
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA045105
Antibody HPA051935
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
14.1
14.1
Antibody dilution
1:250
1:390
PROTEIN ARRAY
Antibody HPA045105
Antibody HPA051935
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
