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Antibody staining overlaps with antibody HPA011922.
Validated
Antibody staining overlaps with antibody HPA007476.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Antibody dilution
1:45
1:35
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA007476
Antibody HPA011922
Standard validation
Supported
Figure description
Immunohistochemical staining of human heart muscle shows cytoplasmic and membranous positivity in myocytes.
Expression
RNA: detected in 15 tissues Protein: detected in 2 cell types
Retrieval
HIER pH6
Antibody dilution
1:900
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA007476
Antibody HPA011922
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424852)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424852)
Target mass (kDa)
32.4
32.4
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA007476
Antibody HPA011922
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:3000
ANTIGEN INFORMATION
Antibody HPA007476
Antibody HPA011922
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
101
95
Antigen sequence
YAKEIHSRVDSSLLLQSTQNVTVNARNSEGEVTGRLKVGPKMVEVQNQQF
QINSNDGKPLFTVDEKEVVVGTDKLRVTGPEGALFEHSVETPLVRADPFQ
D
