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Immunofluorescent staining of human cell line CACO-2 shows localization to nucleus & nucleoli.
Antibody dilution
1:119
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA035638
Antibody HPA063701
Standard validation
Approved
Figure description
Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 75 cell types
Retrieval
HIER pH6
Antibody dilution
1:600
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035638
Antibody HPA063701
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415502)
Target mass (kDa)
14.6
14.6
Antibody dilution
1:250
1:400
PROTEIN ARRAY
Antibody HPA035638
Antibody HPA063701
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
