CORO7-PAM16 - Antibodies - The Human Protein Atlas

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CORO7-PAM16

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA041657

Antibody HPA053586

Antibody HPA062721

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA041657

HPA053586

HPA062721

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

CORO7 - ENSG00000262246 [100%]

PAM16 - ENSG00000217930 [100%]

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA041657, RRID:AB_10796351

Atlas Antibodies Cat#HPA053586, RRID:AB_2682201

Atlas Antibodies Cat#HPA062721, RRID:AB_2684849

VALIDATION SUMMARY

Antibody HPA041657

Antibody HPA053586

Antibody HPA062721

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA041657

Antibody HPA053586

Antibody HPA062721

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, mitochondria & microtubules.

Antibody dilution

1:33

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA041657

Antibody HPA053586

Antibody HPA062721

Standard validation

Uncertain

Figure description

Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.

Expression

RNA: detected in 0 tissues
Protein: detected in 46 cell types

RNA: detected in 0 tissues
Protein: detected in 22 cell types

Retrieval

HIER pH6

Antibody dilution

1:250

1:35

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA041657

Antibody HPA053586

Antibody HPA062721

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

114.2, 100.6, 98.6, 91.7

114.2, 100.6, 98.6, 91.7, 22.2, 22.1, 17.8, 17.7

114.2, 15.9, 15.1, 13.8

Antibody dilution

1:250

1:80

1:110

PROTEIN ARRAY

Antibody HPA041657

Antibody HPA053586

Antibody HPA062721

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

1:1200

ANTIGEN INFORMATION

Antibody HPA041657

Antibody HPA053586

Antibody HPA062721

Antigen

Recombinant protein fragment

Length (aa)

101

Antigen sequence

TRVFLYELLPESPFFLECNSFTSPDPHKGLVLLPKTECDVREVELMRCLR
LRQSSLEPVAFRLPRVRKEFFQDDVFPDTAVIWEPVLSAEAWLQGANGQP
W

RFRVSKFRHTEARPPRRESWISDIRAGTAPSCRNHIKSSCSLIAFNSDRP
GVLGIVPLQGQGEDKRRVAHLGCHSDLVTDLDFSPFDDFLLA

VQKNYEHLFKVNDKSVGGSFYLQSKVVRAKERLDEELKIQAQEDREKGQM
PHT

Matching transcripts

CORO7-PAM16-001 - ENSP00000460885 [100%]

Other gene match

CORO7 - ENSG00000262246 [100%]

PAM16 - ENSG00000217930 [100%]

ANTIGEN VIEW

CORO7-PAM16-001

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.