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ANTIBODY INFORMATION
 
 

Antibody HPA066324

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA066324  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 14  

References

  

Proper citation

 Atlas Antibodies Cat#HPA066324, RRID:AB_2685658  

VALIDATION SUMMARY
 
 

Antibody HPA066324

  
Immunocytochemistry

Supported  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA066324

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.  

Antibody dilution

 1:196  

Literature conformity

 The subcellular location is supported by literature.  

WESTERN BLOT
 
 

Antibody HPA066324

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

 

Figure description

  

Target mass (kDa)

 53.1, 52.4  

Antibody dilution

 1:660  

PROTEIN ARRAY
 
 

Antibody HPA066324

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:7850  

ANTIGEN INFORMATION
 
 

Antibody HPA066324

  

Antigen

 Recombinant protein fragment  

Length (aa)

 70  

Antigen sequence

 
EWVDKNRLALTKTVKDAVQKNSEKYLSELAEQPERKITRNQKRKHDEINH
VQKTYAEMDPTTAALEKEHE
 

Matching transcripts

 KAT8-001 - ENSP00000456933 [100%]
KAT8-002 - ENSP00000406037 [100%]
KAT8-201 - ENSP00000219797 [100%]
 

Other gene match

  
ANTIGEN VIEW
KAT8-001
KAT8-002
KAT8-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.