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ANTIBODY INFORMATION
 
 

Antibody HPA026488

  

Antibody HPA028425

  

Antibody CAB011203

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   SIGMA
  

Product name

 HPA026488   HPA028425   C7863  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 5   6   3  

References

   1    

Proper citation

 Atlas Antibodies Cat#HPA026488, RRID:AB_1847273   Atlas Antibodies Cat#HPA028425, RRID:AB_10600899   Sigma-Aldrich Cat#C7863, RRID:AB_259070  

VALIDATION SUMMARY
 
 

Antibody HPA026488

  

Antibody HPA028425

  

Antibody CAB011203

  
Immunocytochemistry

  Approved    
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA026488

  

Antibody HPA028425

  

Antibody CAB011203

  

Standard validation

   Approved    

     

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.    

Antibody dilution

   1:100    

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA026488

  

Antibody HPA028425

  

Antibody CAB011203

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.   Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in non-germinal center cells.   Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 27 cell types		   RNA: detected in 37 tissues
Protein: detected in 40 cell types		   RNA: detected in 37 tissues
Protein: detected in 50 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:1200   1:1500  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA026488

  

Antibody HPA028425

  

Antibody CAB011203

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 50.7, 12.3   50.7   50.7, 12.3, 8.1, 7.1  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA026488

  

Antibody HPA028425

  

Antibody CAB011203

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:6000    

ANTIGEN INFORMATION
 
 

Antibody HPA026488

  

Antibody HPA028425

  

Antibody CAB011203

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 66   80    

Antigen sequence

 
QDLPFCKGDVLTIVAVTKDPNWYKAKNKVGREGIIPANYVQKREGVKAGT
KLSLMPWFHGKITREQ
  
WSFGILLWEIYSFGRVPYPRIPLKDVVPRVEKGYKMDAPDGCPPAVYEVM
KNCWHLDAAMRPSFLQLREQLEHIKTHELH
  

											 
																				

																		

										
											
Matching transcripts

												
																					CSK-001 - ENSP00000220003 [100%] 
CSK-002 - ENSP00000414764 [100%] 
CSK-006 - ENSP00000454906 [100%] 
CSK-016 - ENSP00000457714 [100%] 

											 
																						CSK-001 - ENSP00000220003 [100%] 
CSK-002 - ENSP00000414764 [100%] 
CSK-006 - ENSP00000454906 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CSK-001
CSK-002
CSK-006
CSK-016
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA026488

												
										 
																				
											
											
											
Antibody HPA028425

												
										 
																				
											
											
											
Antibody CAB011203

												
										 
																		

																	

										
											
1.

												
																					
											 
																							Beta-catenin inhibits melanocyte migration but induces melanoma metastasis

	Gallagher SJ et al

	Oncogene 2013;32(17):2230-8

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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