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ACSBG1

ANTIBODY INFORMATION
 
 

Antibody HPA041642

  

Antibody HPA058869

  

Antibody CAB045955

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Origene
  

Product name

 HPA041642   HPA058869   TA501176  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 12   16   12  

References

      

Proper citation

 Atlas Antibodies Cat#HPA041642, RRID:AB_2677593   Atlas Antibodies Cat#HPA058869, RRID:AB_2683840   OriGene Cat#TA501176, RRID:AB_11124196  

VALIDATION SUMMARY
 
 

Antibody HPA041642

  

Antibody HPA058869

  

Antibody CAB045955

  
Immunocytochemistry

  Approved    
Immunohistochemistry

Approved     Approved  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA041642

  

Antibody HPA058869

  

Antibody CAB045955

  

Standard validation

   Approved    

     

Figure description

   Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleus & vesicles.    

Antibody dilution

   1:79    

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041642

  

Antibody HPA058869

  

Antibody CAB045955

  

Standard validation

 Approved     Approved  

     

Figure description

 Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells.     Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in astrocytes.  

Expression

 RNA: detected in 9 tissues
Protein: detected in 64 cell types		     RNA: detected in 9 tissues
Protein: detected in 38 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:300     1:800  

Literature conformity

 No avaliable gene/protein characterization data.     No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.     Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041642

  

Antibody HPA058869

  

Antibody CAB045955

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 81.3   81.3, 53.9   81.3, 53.9, 27.6, 20.5, 15.7  

Antibody dilution

 1:250   1:200   1:500  

PROTEIN ARRAY
 
 

Antibody HPA041642

  

Antibody HPA058869

  

Antibody CAB045955

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3200    

ANTIGEN INFORMATION
 
 

Antibody HPA041642

  

Antibody HPA058869

  

Antibody CAB045955

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 76   76    

Antigen sequence

 
ANVIMVDTQKQLEKILKIWKQLPHLKAVVIYKEPPPNKMANVYTMEEFME
LGNEVPEEALDAIIDTQQPNQCCVLV
  
TLKCTLDPDTSDQTDNLTEQAVEFCQRVGSRATTVSEIIEKKDEAVYQAI
EEGIRRVNMNAAARPYHIQKWAILER
  

											 
																				

																		

										
											
Matching transcripts

												
																					ACSBG1-001 - ENSP00000258873 [100%] 

											 
																						ACSBG1-001 - ENSP00000258873 [99%] 
ACSBG1-010 - ENSP00000453451 [99%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ACSBG1-001
ACSBG1-010
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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