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IGDCC4

ANTIBODY INFORMATION
 
 

Antibody HPA008510

  

Antibody HPA008576

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA008510   HPA008576  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 16   3  

References

    

Proper citation

   Atlas Antibodies Cat#HPA008576, RRID:AB_1079496  

VALIDATION SUMMARY
 
 

Antibody HPA008510

  

Antibody HPA008576

  
Immunocytochemistry

  Approved  
Immunohistochemistry

  Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA008510

  

Antibody HPA008576

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.  

Antibody dilution

   1:4  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA008510

  

Antibody HPA008576

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human placenta shows moderate cygtoplasmic positivity in trophoblastic cells.  

Expression

   RNA: detected in 17 tissues
Protein: detected in 60 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:75  

Literature conformity

   No avaliable gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.  
 

Standard validation

 Approved    

   

Figure description

 Synaptic staining mainly in the caudate putamen, nucleus accumbens. Strong immunoreactivity in the island of Calleja.    

WESTERN BLOT
 
 

Antibody HPA008510

  

Antibody HPA008576

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 134.2   134.2  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA008510

  

Antibody HPA008576

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA008510

  

Antibody HPA008576

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 137   137  

Antigen sequence

 
VEDRAEVHSLMGGGVSEGRSHSKRKISWAQPSGLSWAGSWAGCELPQAGP
RPALTRALLPPAGTGQTLLLQALVYDAIKGNGRKKSPPACRNQVEAEVIV
HSDFSASNGNPDLHLQDLEPEDPLPPEAPDLISGVGD
  
VEDRAEVHSLMGGGVSEGRSHSKRKISWAQPSGLSWAGSWAGCELPQAGP
RPALTRALLPPAGTGQTLLLQALVYDAIKGNGRKKSPPACRNQVEAEVIV
HSDFSASNGNPDLHLQDLEPEDPLPPEAPDLISGVGD
 

Matching transcripts

 IGDCC4-001 - ENSP00000319623 [100%]
  IGDCC4-001 - ENSP00000319623 [100%]
 

Other gene match

    
ANTIGEN VIEW
IGDCC4-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.