Cell atlas - MCM4 - The Human Protein Atlas

GENERAL INFORMATION
Gene name	MCM4
Gene description	Minichromosome maintenance complex component 4
Protein class	Disease related genes Enzymes Potential drug targets Predicted intracellular proteins
Predicted localization	Intracellular
Number of transcripts	5
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Localized to the nucleoplasm.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Nucleoplasm (validated)
siRNA	The location(s) are Validated based on siRNA mediated gene silencing.
Mouse	Localized to the Nucleoplasm in mouse cells.

DATA RELIABILITY
Reliability score	Validated based on 2 antibodies. HPA004873 , HPA031052
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ASSAYS

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Localized to the nucleoplasm.

Main location

Nucleoplasm (validated)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location
HPA031052	A-431	Nucleoplasm
HPA031052	A549	Nucleoplasm
HPA031052	U-2 OS	Nucleoplasm
HPA004873	A-431	Nucleoplasm
HPA004873	U-2 OS	Nucleoplasm
HPA004873	U-251 MG	Nucleoplasm

siRNA VALIDATION

Antibody

HPA031052

siRNA validation

Validated: Significant downregulation > 25% by both siRNA:s.

Analysis performed in

Nuclear region of segmented cells in 10x-images

siRNA 1

siRNA 2

RFI

35%

34%

p-value

< 0.01

Antibody

HPA004873

siRNA validation

Validated: Significant downregulation > 25% by one siRNA.

Analysis performed in

Nuclear region of segmented cells in 10x-images

siRNA 1

RFI

74%

p-value

< 0.01


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Sample	Cell line
HPA031052	siRNA 1	U-2 OS
HPA031052	siRNA 2	U-2 OS
HPA031052	scrambled	U-2 OS
HPA004873	siRNA 1	U-2 OS
HPA004873	scrambled	U-2 OS

MOUSE CELLS

Main location

Nucleoplasm

Mouse gene

ENSMUSG00000022673 (version 83)


	Toggle channels	Low High G1 S G2 M N/A

Thumbnail	Antibody	Cell line	Location
	HPA031052	NIH 3T3	Nucleoplasm

GENE INFORMATION
Gene name	MCM4 (HGNC Symbol)
Synonyms	CDC21, CDC54, hCdc21, MGC33310, P1-Cdc21
Description	Minichromosome maintenance complex component 4 (HGNC Symbol)
Entrez gene summary	The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
Chromosome	8
Cytoband	q11.21
Chromosome location (bp)	47960185 - 47978160
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000104738 (version 83.38)
Entrez gene	4173
UniProt	P33991 (UniProt - Evidence at protein level)
neXtProt	NX_P33991
Antibodypedia	MCM4 antibodies

PROTEIN BROWSER

MCM4-001	MCM4-004	MCM4-008	MCM4-009	MCM4-011

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
MCM4-001 ENSP00000262105 ENST00000262105	P33991 [Direct mapping] DNA replication licensing factor MCM4 Show all	Enzymes ENZYME proteins Hydrolases Predicted intracellular proteins Disease related genes Potential drug targets Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000082 [G1/S transition of mitotic cell cycle] GO:0000278 [mitotic cell cycle] GO:0000784 [nuclear chromosome, telomeric region] GO:0003677 [DNA binding] GO:0003678 [DNA helicase activity] GO:0003697 [single-stranded DNA binding] GO:0004003 [ATP-dependent DNA helicase activity] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0006260 [DNA replication] GO:0006268 [DNA unwinding involved in DNA replication] GO:0006270 [DNA replication initiation] GO:0006271 [DNA strand elongation involved in DNA replication] GO:0016020 [membrane] GO:0042555 [MCM complex] Show all	863 aa 96.6 kDa	No	0
MCM4-004 ENSP00000430194 ENST00000523944	P33991 [Direct mapping] DNA replication licensing factor MCM4 Show all	Enzymes ENZYME proteins Hydrolases Predicted intracellular proteins Disease related genes Potential drug targets Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000082 [G1/S transition of mitotic cell cycle] GO:0000278 [mitotic cell cycle] GO:0000784 [nuclear chromosome, telomeric region] GO:0003677 [DNA binding] GO:0004003 [ATP-dependent DNA helicase activity] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0006260 [DNA replication] GO:0006270 [DNA replication initiation] GO:0006271 [DNA strand elongation involved in DNA replication] GO:0016020 [membrane] GO:0042555 [MCM complex] Show all	863 aa 96.6 kDa	No	0
MCM4-008 ENSP00000430329 ENST00000518221	E5RFR3 [Direct mapping] DNA replication licensing factor MCM4 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		141 aa 14.8 kDa	No	0
MCM4-009 ENSP00000428833 ENST00000519170	E5RFJ8 [Direct mapping] DNA replication licensing factor MCM4 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005654 [nucleoplasm] Show all	212 aa 23.5 kDa	No	0
MCM4-011 ENSP00000427875 ENST00000520637	E5RG31 [Direct mapping] DNA replication licensing factor MCM4 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0003677 [DNA binding] GO:0005524 [ATP binding] GO:0006260 [DNA replication] Show all	279 aa 31.7 kDa	No	0