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Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Antibody dilution
1:81
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA002907
Antibody HPA005835
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in a granular pattern in hepatocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 69 cell types
RNA: detected in 37 tissues Protein: detected in 72 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:500
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Consistent with RNA expression data.
Standard validation
Approved
Figure description
Neuronal somata and processes are the predominant type of cells stained. However, in parts of the forebrain (mainly hippocampus/striatum) a few blood vessels together with astrocyte-like structures are positive too.
WESTERN BLOT
Antibody HPA002907
Antibody HPA005835
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
35.9, 35.8, 29.1, 19.8
35.8
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA002907
Antibody HPA005835
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
