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HNRNPL

ANTIBODY INFORMATION
 
 

Antibody HPA051748

  

Antibody HPA052661

  

Antibody CAB016326

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA051748   HPA052661   sc-32317  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

 AC104534.3 - ENSG00000268083 [100%]
      

Released in version

 12   13   4  

References

      

Proper citation

 Atlas Antibodies Cat#HPA051748, RRID:AB_2681588   Atlas Antibodies Cat#HPA052661, RRID:AB_2681905   Santa Cruz Biotechnology Cat#sc-32317, RRID:AB_627736  

VALIDATION SUMMARY
 
 

Antibody HPA051748

  

Antibody HPA052661

  

Antibody CAB016326

  
Immunocytochemistry

  Supported   Supported  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA051748

  

Antibody HPA052661

  

Antibody CAB016326

  

Standard validation

   Supported   Supported  

     

Figure description

   Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.  

Antibody dilution

   1:55   1:87  

Literature conformity

   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA051748

  

Antibody HPA052661

  

Antibody CAB016326

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.   Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.   Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 79 cell types		   RNA: detected in 37 tissues
Protein: detected in 79 cell types		   RNA: detected in 37 tissues
Protein: detected in 80 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:700   1:700   1:350  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA051748

  

Antibody HPA052661

  

Antibody CAB016326

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 64.1, 58.4, 50.6, 35.9   64.1, 58.4, 50.6   64.1, 58.4, 50.6, 24.3, 21.9  

Antibody dilution

 1:300   1:270   1:500  

PROTEIN ARRAY
 
 

Antibody HPA051748

  

Antibody HPA052661

  

Antibody CAB016326

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2350   1:2200    

ANTIGEN INFORMATION
 
 

Antibody HPA051748

  

Antibody HPA052661

  

Antibody CAB016326

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 95   79    

Antigen sequence

 
YKDFSESRNNRFSTPEQAAKNRIQHPSNVLHFFNAPLEVTEENFFEICDE
LGVKRPSSVKVFSGKSERSSSGLLEWESKSDALETLGFLNHYQMK
  
DTWDYTNPNLSGQGDPGSNPNKRQRQPPLLGDHPAEYGGPHGGYHSHYHD
EGYGPPPPHYEGRRMGPPVGGHRRGPSRY
  

											 
																				

																		

										
											
Matching transcripts

												
																					HNRNPL-001 - ENSP00000221419 [100%] 
HNRNPL-002 - ENSP00000470231 [100%] 
HNRNPL-005 - ENSP00000469349 [100%] 

											 
																						HNRNPL-001 - ENSP00000221419 [100%] 
HNRNPL-002 - ENSP00000470231 [100%] 
HNRNPL-005 - ENSP00000469349 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					AC104534.3 - ENSG00000268083 [100%]

											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
HNRNPL-001
HNRNPL-002
HNRNPL-005
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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