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Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA032121 across 72 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA032120 across 72 cell types.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in non-germinal center cells.
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells and strong cytoplasmic staining in non-germinal center cells.
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells
Expression
RNA: detected in 33 tissues Protein: detected in 6 cell types
RNA: detected in 33 tissues Protein: detected in 12 cell types
RNA: detected in 33 tissues Protein: detected in 9 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:175
1:75
1:40
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA032120
Antibody HPA032121
Antibody CAB002492
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
31.7, 30.3, 27.6, 24, 22.6
31.7, 30.3, 27.6, 24, 22.6
31.7, 30.3, 27.6, 24, 22.6, 17.6, 2.9
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA032120
Antibody HPA032121
Antibody CAB002492
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
