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Antibody staining overlaps with antibody HPA035240.
Validated
Antibody staining overlaps with antibody HPA035239.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
Antibody dilution
1:32
1:102
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA035239
Antibody HPA035240
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA035240 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA035239 across 46 cell lines.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human epididymis shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human kidney shows strong luminal membranous positivity in cells in tubules.
Expression
RNA: detected in 37 tissues Protein: detected in 61 cell types
RNA: detected in 37 tissues Protein: detected in 48 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:200
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035239
Antibody HPA035240
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417160)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417160)
Target mass (kDa)
56.6
56.6, 39.4, 36.6, 33.7
Antibody dilution
1:125
1:250
PROTEIN ARRAY
Antibody HPA035239
Antibody HPA035240
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:30000
ANTIGEN INFORMATION
Antibody HPA035239
Antibody HPA035240
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
52
56
Antigen sequence
MVADPPRDSKGLAAAEPTANGGLALASIEDQGAAAGGYCGSRDQVRRCLR
AN
