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PLIN3

ANTIBODY INFORMATION
 
 

Antibody HPA006427

  

Antibody HPA066538

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA006427   HPA066538  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 3   14  

References

 2    

Proper citation

 Atlas Antibodies Cat#HPA006427, RRID:AB_1079289   Atlas Antibodies Cat#HPA066538, RRID:AB_2685687  

VALIDATION SUMMARY
 
 

Antibody HPA006427

  

Antibody HPA066538

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Supported   Supported  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA006427

  

Antibody HPA066538

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to lipid droplets.   Immunofluorescent staining of human cell line A-431 shows localization to lipid droplets.  

Antibody dilution

 1:145   1:141  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA006427

  

Antibody HPA066538

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human small intestine shows strong nuclear and cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human duodenum shows moderate cytoplasmic and nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 71 cell types		   RNA: detected in 37 tissues
Protein: detected in 69 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:900  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA006427

  

Antibody HPA066538

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 47.1, 46.9, 45.8, 31.9   47.1, 46.9, 45.8, 31.9  

Antibody dilution

 1:250   1:470  

PROTEIN ARRAY
 
 

Antibody HPA006427

  

Antibody HPA066538

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:5600  

ANTIGEN INFORMATION
 
 

Antibody HPA006427

  

Antibody HPA066538

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 125   71  

Antigen sequence

 
SLGKLRATKQRAQEALLQLSQALSLMETVKQGVDQKLVEGQEKLHQMWLS
WNQKQLQGPEKEPPKPEQVESRALTMFRDIAQQLQATCTSLGSSIQGLPT
NVKDQVQQARRQVEDLQATFSSIHS
  
DKTKSVVTGGVQSVMGSRLGQMVLSGVDTVLGKSEEWADNHLPLTDAELA
RIATSLDGFDVASVQQQRQEQ
 

Matching transcripts

 PLIN3-001 - ENSP00000221957 [99%]
PLIN3-007 - ENSP00000467803 [99%]
PLIN3-008 - ENSP00000465596 [98%]
PLIN3-005 - ENSP00000468476 [80%]
  PLIN3-001 - ENSP00000221957 [100%]
PLIN3-005 - ENSP00000468476 [100%]
PLIN3-007 - ENSP00000467803 [100%]
PLIN3-008 - ENSP00000465596 [100%]
 

Other gene match

    
ANTIGEN VIEW
PLIN3-001
PLIN3-005
PLIN3-007
PLIN3-008

RELEVANT PUBLICATIONS
 
 

Antibody HPA006427

  

Antibody HPA066538

  

1.

 Lipid droplet-binding protein TIP47 regulates hepatitis C Virus RNA replication through interaction with the viral NS5A protein
Vogt DA et al
PLoS Pathog 2013;9(4):e1003302
Application: ICC-IF 		   

2.

 Septin 9 induces lipid droplets growth by a phosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by HCV
Akil A et al
Nat Commun 2016;7:12203
Application: ICC-IF 		   
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.