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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & vesicles.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.
Antibody dilution
1:10
1:75
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA018999
Antibody HPA049600
Standard validation
Approved
Figure description
Immunohistochemical staining of human hippocampus shows strong nucleolar positivity in neuronal cells.
Expression
RNA: detected in 37 tissues Protein: detected in 62 cell types
Retrieval
HIER pH6
Antibody dilution
1:30
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA018999
Antibody HPA049600
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY414399)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
54.4, 23.5, 17.4, 12
54.4, 29.6
Antibody dilution
1:250
1:380
PROTEIN ARRAY
Antibody HPA018999
Antibody HPA049600
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
