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Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Antibody dilution
1:25
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA031798
Antibody HPA049717
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of squamous epithelium shows distinct positivity in Langerhans cells.
Immunohistochemical staining of human stomach, lower shows strong cytoplasmic and membranous positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 32 cell types
RNA: detected in 37 tissues Protein: detected in 48 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:40
1:35
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA031798
Antibody HPA049717
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
133, 121.1
133, 121.1, 20.6
Antibody dilution
1:250
1:200
PROTEIN ARRAY
Antibody HPA031798
Antibody HPA049717
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
