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Immunofluorescent staining of human cell line A-431 shows localization to nucleus, nucleoli fibrillar center & microtubules.
Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nucleoli fibrillar center.
Antibody dilution
1:100
1:50
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA019071
Antibody HPA022416
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human cerebellum shows cytoplasmic and nucleolar positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows cytoplasmic and nucleolar positivity in Purkinje cells.
Expression
RNA: detected in 37 tissues Protein: detected in 56 cell types
RNA: detected in 37 tissues Protein: detected in 67 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:500
1:100
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019071
Antibody HPA022416
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424120)
Target mass (kDa)
37.4
37.4
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA019071
Antibody HPA022416
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
