ADAP1 - Antibodies - The Human Protein Atlas

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ADAP1

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA007033

Antibody HPA012049

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA007033

HPA012049

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA007033, RRID:AB_1846529

Atlas Antibodies Cat#HPA012049, RRID:AB_1846527

VALIDATION SUMMARY

Antibody HPA007033

Antibody HPA012049

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA007033

Antibody HPA012049

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.

Antibody dilution

1:25

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA007033

Antibody HPA012049

Standard validation

Supported

Figure description

Immunohistochemical staining of human cerebral cortex shows cytoplasmic positivity in neuronal and glial cells.

Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.

Expression

RNA: detected in 33 tissues
Protein: detected in 53 cell types

RNA: detected in 33 tissues
Protein: detected in 30 cell types

Retrieval

HIER pH6

Antibody dilution

1:35

1:10

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA007033

Antibody HPA012049

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

44.9, 43.4, 41, 35.4, 32.7, 28.5

44.9, 43.4, 18.8

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA007033

Antibody HPA012049

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA007033

Antibody HPA012049

Antigen

Recombinant protein fragment

Length (aa)

104

105

Antigen sequence

PKQTEGFRKRWFTMDDRRLMYFKDPLDAFARGEVFIGSKESGYTVLHGFP
PSTQGHHWPHGITIVTPDRKFLFACETESDQREWVAAFQKAVDRPMLPQE
YAVE

LGVFICLSCSGIHRNIPQVSKVKSVRLDAWEEAQVEFMASHGNDAARARF
ESKVPSFYYRPTPSDCQLLREQWIRAKYERQEFIYPEKQEPYSAGYREGF
LWKRG

Matching transcripts

ADAP1-001 - ENSP00000265846 [100%]
ADAP1-002 - ENSP00000414436 [100%]
ADAP1-014 - ENSP00000409129 [100%]
ADAP1-201 - ENSP00000407267 [100%]
ADAP1-202 - ENSP00000442682 [100%]
ADAP1-203 - ENSP00000481154 [100%]
ADAP1-204 - ENSP00000478011 [100%]

ADAP1-001 - ENSP00000265846 [100%]
ADAP1-005 - ENSP00000394973 [100%]
ADAP1-202 - ENSP00000442682 [100%]

Other gene match

ANTIGEN VIEW

ADAP1-001	ADAP1-002	ADAP1-005	ADAP1-014	ADAP1-201	ADAP1-202	ADAP1-203	ADAP1-204

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.