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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Antibody dilution
1:110
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA050678
Antibody CAB012175
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in decidual cells.
Immunohistochemical staining of human kidney shows distinct cytoplasmic positivity in distal tubules.
Expression
RNA: detected in 37 tissues Protein: detected in 72 cell types
RNA: detected in 37 tissues Protein: detected in 51 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:600
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA050678
Antibody CAB012175
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 229, 107, 78, 46, 31, 26, 16.2 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
50.7
50.7, 19.1, 12.1
Antibody dilution
1:550
1:500
PROTEIN ARRAY
Antibody HPA050678
Antibody CAB012175
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
