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AEBP1

ANTIBODY INFORMATION
 
 

Antibody HPA047724

  

Antibody HPA063595

  

Antibody HPA064970

  

Antibody CAB009966

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA047724   HPA063595   HPA064970   sc-32919  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

        

Released in version

 16   14   14   4  

References

        

Proper citation

 Atlas Antibodies Cat#HPA047724, RRID:AB_2680132   Atlas Antibodies Cat#HPA063595, RRID:AB_2685052   Atlas Antibodies Cat#HPA064970, RRID:AB_2685394   Santa Cruz Biotechnology Cat#sc-32919, RRID:AB_2225690  

VALIDATION SUMMARY
 
 

Antibody HPA047724

  

Antibody HPA063595

  

Antibody HPA064970

  

Antibody CAB009966

  
Immunocytochemistry

Approved       Approved  
Immunohistochemistry

  Supported   Supported   Supported  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA047724

  

Antibody HPA063595

  

Antibody HPA064970

  

Antibody CAB009966

  

Standard validation

 Approved       Approved  

       

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.       Immunofluorescent staining of human cell line MCF7 shows localization to vesicles.  

Antibody dilution

 1:60       1:8  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.       The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA047724

  

Antibody HPA063595

  

Antibody HPA064970

  

Antibody CAB009966

  

Standard validation

   Supported   Supported   Supported  

       

Figure description

   Immunohistochemical staining of human duodenum shows moderate positivity in glandular cells.   Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in decidual cells.   Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 15 cell types		   RNA: detected in 37 tissues
Protein: detected in 32 cell types		   RNA: detected in 37 tissues
Protein: detected in 39 cell types		  

Retrieval

   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

   1:75   1:275   1:30  

Literature conformity

   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA047724

  

Antibody HPA063595

  

Antibody HPA064970

  

Antibody CAB009966

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		       Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 130.9, 27.6   130.9   130.9, 27.6   130.9, 82.2, 27.6, 18.1  

Antibody dilution

 1:300   1:100   1:630   1:500  

PROTEIN ARRAY
 
 

Antibody HPA047724

  

Antibody HPA063595

  

Antibody HPA064970

  

Antibody CAB009966

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2400   1:1050   1:7550    

ANTIGEN INFORMATION
 
 

Antibody HPA047724

  

Antibody HPA063595

  

Antibody HPA064970

  

Antibody CAB009966

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 76   71   76    

Antigen sequence

 
PPVKPLLPPLPPDYGDGYVIPNYDDMDYYFGPPPPQKPDAERQTDEEKEE
LKKPKKEDSSPKEETDKWAVEKGKDH
  
LSNNWQNPGEETHVEAREHQPEPEEETEQPTLDYNDQIEREDYEDFEYIR
RQKQPRPPPSRRRRPERVWPE
  
PPVKPLLPPLPPDYGDGYVIPNYDDMDYYFGPPPPQKPDAERQTDEEKEE
LKKPKKEDSSPKEETDKWAVEKGKDH
  

											 
																				

																		

										
											
Matching transcripts

												
																					AEBP1-001 - ENSP00000223357 [100%] 
AEBP1-004 - ENSP00000411277 [92%] 

											 
																						AEBP1-001 - ENSP00000223357 [100%] 

											 
																						AEBP1-001 - ENSP00000223357 [100%] 
AEBP1-004 - ENSP00000411277 [92%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
AEBP1-001
AEBP1-004
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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