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DNMBP

ANTIBODY INFORMATION
 
 

Antibody HPA037878

  

Antibody HPA058216

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA037878   HPA058216  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   16  

References

    

Proper citation

 Atlas Antibodies Cat#HPA037878, RRID:AB_10675622   Atlas Antibodies Cat#HPA058216, RRID:AB_2683640  

VALIDATION SUMMARY
 
 

Antibody HPA037878

  

Antibody HPA058216

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Uncertain    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA037878

  

Antibody HPA058216

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm, nucleoli, cytosol & the Golgi apparatus.  

Antibody dilution

   1:65  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA037878

  

Antibody HPA058216

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 29 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:30    

Literature conformity

 Partly consistent with gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA037878

  

Antibody HPA058216

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 177.3   177.3  

Antibody dilution

 1:250   1:220  

PROTEIN ARRAY
 
 

Antibody HPA037878

  

Antibody HPA058216

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:2600  

ANTIGEN INFORMATION
 
 

Antibody HPA037878

  

Antibody HPA058216

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 99   80  

Antigen sequence

 
TIPSLKEGERLFVCICEFTSQELDNLPLHRGDLVILDGIPTAGWLQGRSC
WGARGFFPSSCVRELCLSSQSRQWHSQSALFQIPEYSMGQARALMGLSA
  
GVEWEMPLATDSPTSDPTEVVNGISSQPQVPFHPNLQKSQYYSTVGGSHP
HSEQYPDLLPLEARTRDYASLPPKRMYSQL
 

Matching transcripts

 DNMBP-001 - ENSP00000315659 [100%]
  DNMBP-001 - ENSP00000315659 [100%]
 

Other gene match

    
ANTIGEN VIEW
DNMBP-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.