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ANTIBODY INFORMATION
 
 

Antibody HPA006385

  

Antibody CAB004611

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA006385   sc-13125  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 3   2  

References

 1    

Proper citation

 Atlas Antibodies Cat#HPA006385, RRID:AB_1080447   Santa Cruz Biotechnology Cat#sc-13125, RRID:AB_671403  

VALIDATION SUMMARY
 
 

Antibody HPA006385

  

Antibody CAB004611

  
Immunocytochemistry


Validated
   Supported  
Immunohistochemistry

Supported   Supported  
Western blot


Validated
   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA006385

  

Antibody CAB004611

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		    

   

Figure description

 Nuclear region of segmented cells in 10x-images    

Antibody dilution

 1:72    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center.  

Antibody dilution

 1:74   1:13  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA006385

  

Antibody CAB004611

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human lymph node shows strong nuclear positivity in germinal and non-germinal center cells.   Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells and lymphoid cells outside reaction centra.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 74 cell types		   RNA: detected in 37 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  
 

Standard validation

 Supported    

   

Figure description

 Nuclear positivity observed in neurons, most abundant in cerebral cortex, olfactory bulb and forebrain.    

WESTERN BLOT
 
 

Antibody HPA006385

  

Antibody CAB004611

  

Formal validation: Genetic
Validated

Downregulation visible in one of two siRNA lanes		    

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		    

Target mass (kDa)

 89.4, 87.4, 84.9    

Loading control

 Total protein image    

Antibody dilution

 1:161    
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 89.4, 87.4, 84.9   89.4, 87.4, 84.9, 23, 17.8  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA006385

  

Antibody CAB004611

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA006385

  

Antibody CAB004611

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 122    

Antigen sequence

 
WKLLSQKEKDAYHKKCDQKKKDYEVELLRFLESLPEEEQQRVLGEEKMLN
INKKQATSPASKKPAQEGGKGGSEKPKRPVSAMFIFSEEKRRQLQEERPE
LSESELTRLLARMWNDLSEKKK
  

											 
																				

																		

										
											
Matching transcripts

												
																					UBTF-001 - ENSP00000345297 [100%] 
UBTF-002 - ENSP00000302640 [100%] 
UBTF-004 - ENSP00000377231 [100%] 
UBTF-005 - ENSP00000390669 [100%] 
UBTF-006 - ENSP00000437180 [100%] 
UBTF-007 - ENSP00000432925 [100%] 
UBTF-008 - ENSP00000435708 [100%] 
UBTF-014 - ENSP00000431539 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
UBTF-001
UBTF-002
UBTF-004
UBTF-005
UBTF-006
UBTF-007
UBTF-008
UBTF-014
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA006385

												
										 
																				
											
											
											
Antibody CAB004611

												
										 
																		

																	

										
											
1.

												
																						UBF complexes with phosphatidylinositol 4,5-bisphosphate in nucleolar organizer regions regardless of ongoing RNA polymerase I activity

	Sobol M et al

	Nucleus ;4(6):478-86

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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