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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Antibody dilution
1:100
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA013550
Antibody HPA014137
Standard validation
Supported
Figure description
Immunohistochemical staining of human retina shows strong cytoplasmic positivity in photoreceptor segments.
Expression
RNA: detected in 22 tissues Protein: detected in 4 cell types
Retrieval
HIER pH6
Antibody dilution
1:250
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
Standard validation
Supported
Figure description
Synaptic positivity observed in caudate putamen. Neuronal cell bodies stained in facial nucleus, medulla.
WESTERN BLOT
Antibody HPA013550
Antibody HPA014137
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
77, 76.6, 72
77, 76.6, 72
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA013550
Antibody HPA014137
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
