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RPS6KB1

ANTIBODY INFORMATION
 
 

Antibody HPA039442

  

Antibody CAB003838

  

Antibody CAB018346

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Epitomics
   Santa Cruz Biotechnology
  

Product name

 HPA039442   1494-1   sc-230  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   mAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Supernatant   Affinity  

Other gene match

      

Released in version

 12   2   4  

References

      

Proper citation

 Atlas Antibodies Cat#HPA039442, RRID:AB_2676510     Santa Cruz Biotechnology Cat#sc-230, RRID:AB_632156  

VALIDATION SUMMARY
 
 

Antibody HPA039442

  

Antibody CAB003838

  

Antibody CAB018346

  
Immunocytochemistry

Supported      
Immunohistochemistry

Approved   Approved   Approved  
Western blot


Validated
   Supported   Supported  
Protein array

Approved      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA039442

  

Antibody CAB003838

  

Antibody CAB018346

  

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.      

Antibody dilution

 1:29      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039442

  

Antibody CAB003838

  

Antibody CAB018346

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human bone marrow shows distinct nuclear positivity in hematopoietic cells.   Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.   Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 50 cell types		   RNA: detected in 37 tissues
Protein: detected in 46 cell types		   RNA: detected in 37 tissues
Protein: detected in 76 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:150   1:65  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA039442

  

Antibody CAB003838

  

Antibody CAB018346

  

Formal validation: Genetic
Validated

Downregulation visible in one of two siRNA lanes		      

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		      

Target mass (kDa)

 59.1, 56.6, 53      

Loading control

 Total protein image      

Antibody dilution

 1:64      
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 59.1, 56.6, 53   59.1, 56.6, 53, 51, 6.9   59.1, 56.6, 53, 51, 6.9  

Antibody dilution

 1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA039442

  

Antibody CAB003838

  

Antibody CAB018346

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA039442

  

Antibody CAB003838

  

Antibody CAB018346

  

Antigen

 Recombinant protein fragment   Synthetic peptide   Synthetic peptide  

Length (aa)

 71      

Antigen sequence

 
VSPVKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTMSGEASAP
LPIRQPNSGPYKKQAFPMISK
     

Matching transcripts

 RPS6KB1-001 - ENSP00000225577 [100%]
RPS6KB1-008 - ENSP00000441993 [100%]
RPS6KB1-201 - ENSP00000376744 [100%]
     

Other gene match

      
ANTIGEN VIEW
RPS6KB1-001
RPS6KB1-008
RPS6KB1-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.