NUP88 - Antibodies - The Human Protein Atlas

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NUP88

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA021816

Antibody CAB002209

Provider

Atlas Antibodies
Sigma-Aldrich

Novocastra

Product name

HPA021816

NCL-Nup88

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA021816, RRID:AB_1854725

VALIDATION SUMMARY

Antibody HPA021816

Antibody CAB002209

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA021816

Antibody CAB002209

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

Antibody dilution

1:7

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA021816

Antibody CAB002209

Standard validation

Approved

Figure description

Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.

Immunohistochemical staining of human stomach shows nuclear membrane positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 73 cell types

RNA: detected in 37 tissues
Protein: detected in 20 cell types

Retrieval

HIER pH6

HIER pH9

Antibody dilution

1:10

1:25

Literature conformity

Consistent with gene/protein characterization data.

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA021816

Antibody CAB002209

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 229, 107, 78, 46, 31, 26, 16.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

83.5, 78.8

83.5, 78.8, 16.9

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA021816

Antibody CAB002209

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

ANTIGEN INFORMATION

Antibody HPA021816

Antibody CAB002209

Antigen

Recombinant protein fragment

Not known

Length (aa)

136

Antigen sequence

QLLTRNVVFGLGGELFLWDGEDSSFLVVRLRGPSGGGEEPALSQYQRLLC
INPPLFEIYQVLLSPTQHHVALIGIKGLMVLELPKRWGKNSEFEGGKSTV
NCSTTPVAERFFTSSTSLTLKHAAWYPSEILDPHVV

Matching transcripts

NUP88-001 - ENSP00000458954 [100%]
NUP88-004 - ENSP00000225696 [93%]

Other gene match

ANTIGEN VIEW

NUP88-001	NUP88-004

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.