RAB5C - Antibodies - The Human Protein Atlas

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RAB5C

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA003426

Antibody HPA004167

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA003426

HPA004167

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA003426, RRID:AB_1079735

Atlas Antibodies Cat#HPA004167, RRID:AB_2667156

VALIDATION SUMMARY

Antibody HPA003426

Antibody HPA004167

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA003426

Antibody HPA004167

Formal validation: Tagged protein

Validated

Antibody staining overlaps with GFP tagged protein

Figure description

Immunofluorescent staining of transgenic HeLa cells show antibody staining in vesicles and GFP expression in vesicles.

Antibody dilution

1:34

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to endosomes.

Antibody dilution

1:34

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA003426

Antibody HPA004167

Standard validation

Approved

Figure description

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.

Expression

RNA: detected in 37 tissues
Protein: detected in 65 cell types

Retrieval

HIER pH6

Antibody dilution

1:50

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA003426

Antibody HPA004167

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.

Figure description

Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

27, 23.5

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA003426

Antibody HPA004167

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA003426

Antibody HPA004167

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

NSLLFMETSAKTAMNVNEIFMAIAKKLPKNEPQNATGAPGRNRGVDLQEN
NPASRSQCCSN

NSLLFMETSAKTAMNVNEIFMAIAKKLPKNEPQNATGAPGRNRGVDLQEN
NPASRSQCCSN

Matching transcripts

RAB5C-001 - ENSP00000345689 [100%]
RAB5C-002 - ENSP00000447053 [100%]
RAB5C-201 - ENSP00000377440 [100%]

Other gene match

ANTIGEN VIEW

RAB5C-001	RAB5C-002	RAB5C-201

RELEVANT PUBLICATIONS

Antibody HPA003426

Antibody HPA004167

Rab5 isoforms differentially regulate the trafficking and degradation of epidermal growth factor receptors
Chen PI et al
J Biol Chem 2009;284(44):30328-38

Identification of components of the host type IA phosphoinositide 3-kinase pathway that promote internalization of Listeria monocytogenes
Jiwani S et al
Infect Immun 2012;80(3):1252-66

Inhibition of cell proliferation and migration by miR-509-3p that targets CDK2, Rac1, and PIK3C2A
Yoon S et al
Mol Cells 2014;37(4):314-21
Application: IHC

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.