LUC7L3 - Antibodies - The Human Protein Atlas

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LUC7L3

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA018475

Antibody HPA018484

Antibody HPA020017

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA018475

HPA018484

HPA020017

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA018475, RRID:AB_1847247

Atlas Antibodies Cat#HPA018484, RRID:AB_1847248

Atlas Antibodies Cat#HPA020017, RRID:AB_1847249

VALIDATION SUMMARY

Antibody HPA018475

Antibody HPA018484

Antibody HPA020017

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA018475

Antibody HPA018484

Antibody HPA020017

Formal validation: Genetic

Validated

Significant downregulation > 25% by one siRNA.

Figure description

Nuclear region of segmented cells in 10x-images

Antibody dilution

1:185

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA018484.

Validated

Antibody staining overlaps with antibody HPA018475.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.

Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.

Antibody dilution

1:34

1:182

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA018475

Antibody HPA018484

Antibody HPA020017

Standard validation

Supported

Figure description

Immunohistochemical staining of human lymph node shows strong nuclear positivity in germinal center and non-germinal center cells.

Immunohistochemical staining of human lymph node shows moderate nuclear positivity in germinal center and non-germinal center cells.

Immunohistochemical staining of human lymph node shows strong nuclear positivity in germinal center and non-germinal center cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 74 cell types

RNA: detected in 37 tissues
Protein: detected in 67 cell types

RNA: detected in 37 tissues
Protein: detected in 81 cell types

Retrieval

HIER pH6

Antibody dilution

1:125

1:3000

1:175

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA018475

Antibody HPA018484

Antibody HPA020017

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

58.2, 51.5, 14.4, 13.3

58.2, 51.5

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA018475

Antibody HPA018484

Antibody HPA020017

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA018475

Antibody HPA018484

Antibody HPA020017

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

RDRKSYKHRSKSRDREQDRKSKEKEKRGSDDKKSSVKSGSREKQSEDTNT
ESKESDTKNEVNGTSEDIKSEGDTQSN

LAEVERRIRRGHARLALSQNQQSSGAAGPTGKNEEKIQVLTDKIDVLLQQ
IEELGSEGKVEEAQGMMKLVEQLKEERELLRSTTSTIESFA

KQMEVCEVCGAFLIVGDAQSRVDDHLMGKQHMGYAKIKATVEELKEKLRK
RTEEPDRDERLKKEKQEREEREKEREREREERER

Matching transcripts

LUC7L3-001 - ENSP00000425092 [100%]
LUC7L3-002 - ENSP00000240304 [100%]
LUC7L3-004 - ENSP00000376919 [92%]
LUC7L3-022 - ENSP00000427927 [92%]
LUC7L3-026 - ENSP00000430407 [92%]

LUC7L3-001 - ENSP00000425092 [100%]
LUC7L3-002 - ENSP00000240304 [100%]
LUC7L3-004 - ENSP00000376919 [100%]

Other gene match

ANTIGEN VIEW

LUC7L3-001	LUC7L3-002	LUC7L3-004	LUC7L3-022	LUC7L3-026

RELEVANT PUBLICATIONS

Antibody HPA018475

Antibody HPA018484

Antibody HPA020017

RRP1B is a metastasis modifier that regulates the expression of alternative mRNA isoforms through interactions with SRSF1
Lee M et al
Oncogene 2014;33(14):1818-27

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.