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YWHAE

ANTIBODY INFORMATION
 
 

Antibody HPA008445

  

Antibody CAB016200

  

Antibody CAB021109

  

Antibody CAB047350

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
   GenWay Biotech
   NCI-CPTC
  

Product name

 HPA008445   sc-23957   15-288-21945F   CPTC-YWHAE-1  

Host species

 Rabbit   Mouse   Hen   Mouse  

Clonality

 pAb   mAb   msAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Not known   Protein A/G  

Other gene match

        

Released in version

 4   4   5   9  

References

 1        

Proper citation

 Atlas Antibodies Cat#HPA008445, RRID:AB_1844336   Santa Cruz Biotechnology Cat#sc-23957, RRID:AB_626619   GenWay Biotech Inc. Cat#15-288-21945F, RRID:AB_10060396    

VALIDATION SUMMARY
 
 

Antibody HPA008445

  

Antibody CAB016200

  

Antibody CAB021109

  

Antibody CAB047350

  
Immunocytochemistry

Approved        
Immunohistochemistry

Approved   Approved   Approved   Approved  
Western blot

Supported   Supported   Supported   Supported  
Protein array

Supported        

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA008445

  

Antibody CAB016200

  

Antibody CAB021109

  

Antibody CAB047350

  

Standard validation

 Approved        

       

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to cytosol.        

Antibody dilution

 1:25        

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.        

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA008445

  

Antibody CAB016200

  

Antibody CAB021109

  

Antibody CAB047350

  

Standard validation

 Approved   Approved   Approved   Approved  

       

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human cerebral cortex shows moderate nuclear and cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.   Immunohistochemical staining of human cerebral cortex shows moderate positivity in neuropil.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 71 cell types		   RNA: detected in 37 tissues
Protein: detected in 66 cell types		   RNA: detected in 37 tissues
Protein: detected in 63 cell types		   RNA: detected in 37 tissues
Protein: detected in 54 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:1000   1:4000   1:10000   1:60000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA008445

  

Antibody CAB016200

  

Antibody CAB021109

  

Antibody CAB047350

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

       

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 29.2, 12.7   29.2, 26.5, 12.9, 12.7, 4.5   29.2, 26.5, 12.9, 12.7, 4.5   29.2, 26.5, 12.9, 12.7, 4.5  

Antibody dilution

 1:250   1:500   1:1000   1:500  

PROTEIN ARRAY
 
 

Antibody HPA008445

  

Antibody CAB016200

  

Antibody CAB021109

  

Antibody CAB047350

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		        

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.        

Antibody dilution

 1:3000        

ANTIGEN INFORMATION
 
 

Antibody HPA008445

  

Antibody CAB016200

  

Antibody CAB021109

  

Antibody CAB047350

  

Antigen

 Recombinant protein fragment   Native protein   Not known   Recombinant protein  

Length (aa)

 70        

Antigen sequence

 
MDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYK
NVIGARRASWRIISSIEQKE
  

											 
																						

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					YWHAE-001 - ENSP00000264335 [100%] 
YWHAE-012 - ENSP00000460712 [100%] 

											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
YWHAE-001
YWHAE-012
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA008445

												
										 
																				
											
											
											
Antibody CAB016200

												
										 
																				
											
											
											
Antibody CAB021109

												
										 
																				
											
											
											
Antibody CAB047350

												
										 
																		

																	

										
											
1.

												
																						14-3-3 fusion oncogenes in high-grade endometrial stromal sarcoma

	Lee CH et al

	Proc Natl Acad Sci U S A 2012;109(3):929-34

			
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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