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Immunohistochemical staining of human kidney shows distinct positivity in plasma.
Immunohistochemical staining of human kidney shows distinct positivity in tubular cells.
Immunohistochemical staining of human kidney shows moderate cytoplasmic and membranous positivity in tubular cells.
Expression
RNA: detected in 27 tissues Protein: detected in 14 cell types
RNA: detected in 27 tissues Protein: detected in 35 cell types
RNA: detected in 27 tissues Protein: detected in 34 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:400
1:600
1:50000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA060933
Antibody CAB016695
Antibody CAB016765
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
54.3, 13
54.3, 13
54.3, 13
Antibody dilution
1:200
1:500
1:500
PROTEIN ARRAY
Antibody HPA060933
Antibody CAB016695
Antibody CAB016765
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
